Hijikata reunites with Izo, now a deadly assassin. After their tragic duel, he forges a ruthless code to protect his remaining brothers.

The murder of a young member pushes Nagakura toward execution. As Kondo sacrifices his pride to save his comrade, a shocking betrayal comes to light.

Outnumbered 150 to 10, Yamanami devises a desperate plan. Harada makes a suicidal last stand, buying time for his brothers-in-arms to strike back.

As Serizawa's violence spirals, Hijikata confronts the man he calls his shadow. They agree to settle everything in one last, deadly clash.

The unit charges headlong into the fray. As comrades fall to clear the path, a wounded Hijikata entrusts their future to the prodigy, Okita.

Okita defeats Serizawa in a final showdown. As the age of swords ends, a new power, Shinsaku Takasugi, emerges before Hijikata and his brothers.